C4482
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 | Figure 1. CelLytic Y vs. Glass Beads Method. One gram of S. cerevisiea washed cell pellet was extracted with the following: CelLytic Y, a competitor’s reagent, or glass beads procedure. Protein was determined using BCA protein assay. |
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C4482
Sigma
CelLytic™ Y Cell Lysis Reagent
For yeast cells
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Features and Benefits | • Fast: Cell lysis in just 20 minutes • Efficient: Three times more efficient than glass beads • Flexible: Lysis is enhanced by the addition of reducing reagent (DTT) • Gentle: Non-denaturing - preserves biological activity |
| General description | CelLytic Y Cell Lysis Reagent is versatile, phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells. |
| Legal Information | CelLytic is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
